In the past decade, rapid growth of Thailand’s economy has affected the pattern of landusage in Ayutthaya city. Large agricultural land areas had become golf courses, gardenhomes, housing areas and industrial estates. This is a result of booming land pricesbetween 1985 to 1990 which has encouraged land owners to sell their land at priceshigher than the real value. In addition, from 1991, an industrial development policy hasmade Ayutthaya as an industrial area and this move has affected the pattern of landusage, land utilization and bring about problems such as national forest reserveencroachment, demolishment of historical heritage, environmental pollution,transportation as well as social problems.This study focuses on the impact of industrial development on urban land usage inAyutthaya, Thailand. The study aims to investigate the pattern of land usage, analysethe rate of change on land utilization and determine the effect of industrial developmenton land usage and quality of life. The data were collected using questionnaires and secondary resources. The study covered Ayutthaya province in three areas; PhraNakhon Si Ayutthaya, Sena and Uthai. The sample frame includes people who areinvolved in agricultural and industrial sectors in Ayutthaya. The sample size includedmembers from 500 households. The data were analysed using SPSS program andstatistical analysis such as ANOVA, regression, chi-squre, and t-test were used forhypotheses testing.The findings showed that land were used in two different ways in Ayutthaya; (1) farmholding land which is used for agriculture and rearing livestock, and (2) land which isused for residential, business, commercial, and industrial activities. The rate of changefor land utilization indicated that the amount of agricultural areas are decreasing due tothe establishment of industrial estates, increasing number of factories and changes tothe land usage pattern. Lastly, industrial development is also affecting the lifestyles ofthe surrounding population.